Fluorescence in Situ Hybridization and Transmission Electron Microscopy in Spermatozoa from Patients with High FSH Levels
نویسندگان
چکیده
The chromosomal aneuploidies were analyzed even in the sperm extracted from testis with regard to the different conditions determining azoospermia including groups with nonobstructive azoospermia and in severe oligozoospermia with high Follicle Stimulating Hormone (FSH) levels [6]. To the best of our knowledge, reports showing values of sperm aneuploidies in presence of high FSH levels are lacking. On the other hand, the effect of elevated FSH levels in the development of aneuploidies in oocytes is better documented [7,8].
منابع مشابه
Accuracy Assessment of Interphase Fluorescence In-Situ Hybridization on Uncultured Amniotic Fluid Cells
a:4:{s:10:"Background";s:431:"Parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. It seems that the implementation of faster techniques such as fluorescence in-situ hybridization (FISH) will have some benefits in reducing this anxiety. Besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for tre...
متن کاملRetrospective assessment of radiation dose by Fluorescence In Situ Hybridization and evaluation of stable chromosomal aberrations
Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose,...
متن کامل18, X, Y aneuploidies and transmission electron microscopy studies in spermatozoa from five carriers of different reciprocal translocations.
We analysed ejaculated spermatozoa from five infertile men with different balanced reciprocal translocations to contribute to the study of meiotic segregation of chromosomes 18, X and Y and also to evaluate sperm morphology by transmission electron microscopy (TEM) analysis. Conventional lymphocyte karyotype analyses highlighted different reciprocal balanced translocations: t(12;13), t(4;9), t(...
متن کاملAssessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles.
BACKGROUND Spermatozoa with large vacuoles (SLV) may have a negative impact on embryo development. The origin of these vacuoles is unknown. We evaluated acrosome and nucleus alterations in isolated SLV, versus unselected spermatozoa. METHODS We studied 20 patients with teratozoospermia. Spermatozoa from the native semen sample and spermatozoa presenting a vacuole occupying >13.0% total head a...
متن کاملSuccessful childbirth after intracytoplasmic morphologically selected sperm injection without assisted oocyte activation in a patient with globozoospermia.
We here report a successful pregnancy and healthy childbirth obtained in a case of total globozoospermia after intracytoplasmic morphologically selected sperm injection (IMSI) without assisted oocyte activation (AOA). Two semen analyses showed 100% globozoospermia on classic spermocytogram. Motile sperm organelle morphology examination (MSOME) analysis at ×10,000 magnification confirmed the rou...
متن کامل